Sysmex公司XN-1000v全自動(dòng)動(dòng)物血液分析儀在魚類上的應(yīng)用
Abstract
Hematological studies provide essential information about the health of animals, which is crucial for veterinary medicine, scientific research, and aquaculture. Automatic hematological analyzers are an alternative to manual methods, offering faster and more reliable results. The objective of this study was to validate the Sysmex XN-1000V automatic hematology analyzer for blood samples from rainbow trout (Oncorhynchus mykiss), examine the effects of two anticoagulants (K2EDTA and lithium heparin), and establish normal blood reference values for this fish species. Additionally, comparative studies were conducted between the Sysmex XN-1000V and manual methods (hemocytometer cell count and blood smear estimation), and reference intervals were established. Ninety-nine heparinized blood samples were analyzed for validation and sample stability tests. The results showed extremely good precision, with a coefficient of variation (CV) below 3% for RBCs, HGB, and HCT and less than 5% for non-RBC cells (leukocytes plus thrombocytes). However, heterophils (%) exhibited higher variability, with a CV of 15.08%. Linearity was excellent, and the carry-over was below 1% for all parameters. The sample stability test indicated that samples could be analyzed for up to 48 h when stored at 4 ?C and up to 24 h at room temperature. Non-RBC cells were the first to degrade over time. The automated and manual methods demonstrated good correlation and agreement, validating the analyzer’s accuracy. The effects of two anticoagulants, K2EDTA and lithium heparin, on the blood samples were also studied. Heparin was the preferred anticoagulant for routine hematological analysis of rainbow trout blood with the Sysmex XN-1000V analyzer. In conclusion, the Sysmex XN-1000V enables complete hemogram analyses to be performed quickly and accurately, standardizing techniques, harmonizing results, and providing reliable reference intervals with O mykiss blood.
摘要：
血液學(xué)研究提供了有關(guān)動(dòng)物健康的重要信息，這對(duì)獸醫(yī)、科學(xué)研究和水產(chǎn)養(yǎng)殖至關(guān)重要。自動(dòng)血液分析儀是手動(dòng)方法的替代品，可提供更快、更可靠的結(jié)果。本研究的目的是驗(yàn)證Sysmex XN-1000V全自動(dòng)血液分析儀對(duì)虹鱒（Oncorhynchus mykiss）血液樣本的檢測(cè)效果，檢查兩種抗凝劑（K2EDTA和肝素鋰）的效果，并為該魚類建立正常的血液參考值。此外，對(duì)Sysmex XN-1000V和手動(dòng)方法（血細(xì)胞計(jì)數(shù)器細(xì)胞計(jì)數(shù)和血涂片估計(jì)）進(jìn)行了比較研究，并建立了參考區(qū)間。對(duì)99份肝素化血液樣本進(jìn)行了驗(yàn)證和樣本穩(wěn)定性測(cè)試分析。結(jié)果顯示，精確度極高，紅細(xì)胞、血紅蛋白和紅細(xì)胞壓積的變異系數(shù)（CV）低于3%，非紅細(xì)胞（白細(xì)胞和血小板）的變異系數(shù)低于5%。然而，異質(zhì)性（%）表現(xiàn)出更高的變異性，變異系數(shù)為15.08%。線性非常好，所有參數(shù)的結(jié)轉(zhuǎn)率均低于1%。樣品穩(wěn)定性測(cè)試表明，在4℃下儲(chǔ)存時(shí)，樣品可以分析長(zhǎng)達(dá)48小時(shí)?在室溫下持續(xù)24小時(shí)。非紅細(xì)胞是第一個(gè)隨著時(shí)間的推移而降解的細(xì)胞。自動(dòng)和手動(dòng)方法顯示出良好的相關(guān)性和一致性，驗(yàn)證了分析儀的準(zhǔn)確性。還研究了兩種抗凝劑K2EDTA和肝素鋰對(duì)血液樣本的影響。肝素是Sysmex XN-1000V分析儀對(duì)虹鱒血進(jìn)行常規(guī)血液學(xué)分析的首選抗凝劑。總之，Sysmex XN-1000V能夠快速準(zhǔn)確地進(jìn)行完整的血象分析，規(guī)范技術(shù)，協(xié)調(diào)結(jié)果，并為O mykiss血液提供可靠的參考區(qū)間。
關(guān)鍵詞：XN-1000v全自動(dòng)動(dòng)物血液分析儀，魚類健康；血液學(xué)；血液學(xué)方法驗(yàn)證；血液自動(dòng)分析儀；虹鱒魚
